Leica application suite x las x is the one software platform for all leica microscopes. Up to eight excitation lines can be used simultaneously. Leica sp8 confocal with white light laser, falcon flim. Leica sp8 confocal with lightning deconvolution currently selected leica sp8 confocal with white light laser, falcon flim, sted and deconvolution zeiss z. Leica sp8 white light confocal, gsted, flim cell sciences. Software to process, model and manage 3d point clouds. Leica application suite x microscope software youtube. Digital reality plugins for your favourite cad systems. Flexible automatic deformation monitoring software solution. Turn the pc microscope, scanner power, laser power, and the laser emission key to on bottom right of desk. Leica sp8 user manuals and brochures download the leica tcs sp8 quick start poster and cores abridged instructions for getting started here. In order to keep information uptodate, a description of software functions. Top 4 download periodically updates software information of leica full versions from the publishers, but some information may be slightly outofdate using warez version, crack, warez passwords, patches, serial numbers, registration codes, key generator, pirate key, keymaker or keygen for leica license key is illegal. The system then uses automatic onboard software panel stitching to assemble the final image.
Leicas microscope software makes imaging and documentation easy. Leica application suite x las x is the easy to use software platform for advanced life science research on leica microsystems confocal, widefield. The leica sp8 confocal microscope is on a leica dmi8 inverted microscope stand. From basic documentation to advanced life science research las x directly navigates you to brilliant imaging. For example, you can start with a compact system with few solid state line lasers and evolve to a leica tcs sp8 x with white light laser at a later time. Features and highlights white light laser with aobs provides super continuous excitation optimally excite any fluorophore in the 470nm670nm excitation range. It integrates confocal, widefield, stereo, superresolution, and lightsheet instruments from leica microsystems. This system can achieve a resolution of 50 nm or less. The components are the microscope body, two automated stages, the scan head, the electrooptical modulator eom, the monitor and computer, and the spectraphysics maitai tunable nearir laser.
How to update the firmware on the leica t red dot forum. Further help is available via inprogram help within the leica lasaf software. Commonly, this programs installer has the following filename. The leica sp8 x is a high sensitivity confocal microscope system that offers complete flexibility for any fluorescence experiment. Complex experimental designs, 3d image tiling and 3d rendering and measurements are possible with the las af leica software. Ladapter leica accessories lmount ssystem cameras ssystem lenses slsystem cameras slsystem lenses msystem cameras msystem lenses msystem equipment q camera. Leica las x microscope software mobile connection youtube. With the buttons load and save in the sequential scan dialog, you can load a previously saved sequential scan setting or save the current setting note. The leica confocal microscope has been upgraded with a 3d sted superresolution system.
Leica las af lite is a platform designed for advanced life science research with leica microsystems confocal and widefield systems. To open the images you can either use the leica las x core software windows only. It integrates confocal, widefield, stereo, superresolution, and lightsheet instruments. Leica sp8 tcs users manual 6 double click on las x software can take up to two minutes for software to launch. Today, leica camera has announced a new firmware update for the leica t typ 701 at photokina. Freely tunable confocal imaging with the leica tcs sp8 x the white light laser wll is the optimal excitation source to image any kind of dye combination. Leica sted 3x quick reference sheet acrf cancer biology. The leica tcs sp8 is a fully motorised highresolution spectral confocal system. Equipment molecular cytology core university of missouri. If you are the last user fo the day, turn off the complete system incl. Leica lasx is the standard software used for the leica microscopes. The leica sp8 confocal microscope is for live cell imaging with highspeed, multispectral, supersensitivity, superresolution, and fluorescence lifetime capability. Other than using leica biosystems directly, the following company is the only other leica biosystems north america authorized service provider, for selected products.
Upon deconvolution, a clearly enhanced definition of sympathetic. Turn on the fluorescent lamp top left of the computer monitor. Leica spe confocal microscope harvard neurodiscovery center. Fill out the logbook and comment on the performance of the system or failure description. Leica sp8 x confocal microscope harvard neurodiscovery center. View and download leica sp8 training manual online. A message will appear asking whether you want to initialize the stage.
User manual leica tcs sp8 leica tcs sp8 mp leica tcs sp8 x. Leica sp8 inverted scanning confocal microscope imaging. Page 5 shutdown procedure turn off the lasers in the software 2. Download free las x core offline version for life science download leica. Microscope software platform las x for all leica microscopes. Magazine the world of leica photography since 1949. Leica sp8 pointscanning confocal system, on a leica dmi6000b inverted microscope, confocal laser scanning microscope leica tcs sp8 en, pdf, 535. Leica application suite x las x is the easy to use software platform for advanced life science research on leica microsystems confocal. This system has a tandem scanner that has both a standard scanner lower speed, resolution up to 8k x 8k and a resonant scanner higher speed, resolution up to 1k x 1k. Very handy for new users who dont want to download individual plugins. Lower the objective to the lowest position, remove specimen and clean all of the objectives 3. The leica tcs sp8 is a fully motorised highresolution spectral confocal system, based on a leica dmi8 microscope, with.
Downloads brochures, firmware, technical data, instructions, images and more. Theres also the full leica sp8 user manual plus the other leica sp8 confocal manuals. Leica application suite free version download for pc. Page 6 the las x software will open in the acquire tab.
To understand their coverage area and authorized products, you may contact us or them directly. Leica sp8 2photon dive duke light microscopy core facility. Leica microsystems cms gmbh am friedensplatz 3 d68165 mannheim germany. Double click on the las x software found on the desktop. We have a leica sp8 confocal with white light laser. Software and standard operating procedure leica application suite x download free las x core viewer here standard operating procedure. Leica confocal software lcs lite free download windows. Free software cell and developmental biology microscopy core. We also do not have 2p on this system, but the leica software is laid out in a similar. Life sciences are continually advancing and your research changes accordingly. High speed high resolution optical sectioning and 3d microscopy. Sp8 lightning confocal microscope products leica microsystems. Leica tcs sp8 confocal microscope biological imaging.
Download the information on the cell culture dishesor chambers for leica sp8 confocal microscopy here. Free software from leica to open images acquired on the leica sp8 confocal or any microscope controlled by las x. Free software cell and developmental biology microscopy. Leica sp8 confocal microscope ross fluorescence imaging center. When in doubt, turning the scanner off and on again and restarting the software is a good idea. Review your experiment or download a specific image in full resolution and send it to a colleague. Live cell imaging with adaptive focus control afc to automatically maintain focus in timelapse experiments. Navigator software module creates a fast overview of samples. By tuning both excitation and detection, complete excitation emission spectra can be acquired with this spectral information, any dye can be optimally excited with minimum crossexcitation and specimen damage. Confocal imaging using a super continuum, white light wll pulsed laser source that allows users to specify any excitation wavelength between 470nm and 670nm. Leica confocal software lcs lite free download windows version. Ernest mario school of pharmacy, william levine hall, room 002. Leica tcs sp8 wll confocal smd microscope wellcome. Leica sp8 confocal with lightning deconvolution leica sp8 confocal with white light laser, falcon flim, sted and deconvolution currently selected zeiss z.
Together with an aobs acoustical optical beam splitter up to 8 discrete laser lines can be selected simultaneously with a 1 nm precision. Leica tcs sp8 technical documentation en 11 2016, pdf, 499. View and download leica tcs sp8 quick start manual online. Las x life science microscope software platform products. In addition to standard pmts this system has hyd detectors gaasp that combine highsensitivity and low. Leica sp8 tcs users manual follow the procedure for start up and log on as posted in the lab. Look at the bottom of the page on the leica website for download. Free software from leica to open images acquired on the leica sp8 confocal or any. From basic documentation to advanced life science research las x.
Leica microsystems is your longterm partner to help you grow your confocal equipment as needed. Here is an example of a basic frap experiment using the leica sp8. When the leica software restarts, it will ask you if you want the data it found. Leica sp8 tcs users manual v2 university of oklahoma. Leica tcs sp8 confocal microscope flow cytometrycell. I agree with claus the system performance is very good. Brochures, firmware, technical data, instructions, images and more. Leica tcs sp8 wll confocal smd microscope wellcome centre. The protein is unbound in the cytoplasm, therefore fluorescence should return to a photobleached region via diffusion. If there will be another user within 3 hours, your only responsibilities will be to shut down the software step 6 and indicate in the logbook that you have left the system on. Microscope imaging software products leica microsystems. Download links are directly from our mirrors or publishers website, leica. Leica sp8 confocal with white light laser, falcon flim, sted and deconvolution.
Three depletion lasers 592, 660 and 775 nm enable multicolor detection of a. Sp8 lightning is a modular confocal microscope designed for optimal photon efficiency and high speed. Leica application suite the new leica application suite integrates leica automated microscopes, digital cameras and software leica application suite solves and accelerates routine and research analysis. Leica ssystem a class of its own leica s3 64 megapixel. User guide to the ibif leica tcs sp8 mp confocal microscope. This free program is a product of leica microsystems hd gmbh am friedensplatz 3 d68165 mannheim. If you select yes, the stage is going to move to all 4 corners to calibrate the position of the stage. How can i export pic as tiff on las af leica software. The specimen is a fly brain explant, expressing a yfptagged protein in the mushroom bodies. I have done if experiment using four colours red, green, blue and grey, and i would like to merge only three of them. Scroll down the linked page to find the version appropriate to your operating system.
The instrument is a leica tcs sp8 mp confocal microscope, equipped for multiphoton confocal microscopy. You can expand any menu on the left part of the settings screen by clicking on the black arrows. This is an inverted microscope with the objectives below the specimen. The white light laser source of the leica tcs sp8 x perfectly matches the wavelength of any fluorophore. Leica infinity geospatial office software suite is designed to manage, process, analyse and quality check field survey data from uavs and terrestrial surveying equipment. Leica tcs sp8 option machineposter en pdf, 728 kb sp8 scan head with 4tuneposter rgb may2018.
Jun 09, 2015 leica application suite x las x is the easy to use software platform for advanced life science research on leica microsystems confocal, widefield and superresolution systems. Changing hardware parameters such as the detection bandwidth, the excitation beam splitter, the laser shutter or the. Leica biosystems provides high quality support to our customers. For example, if you want to collect 3d image stacks, select xyz from the dropdown. Leica sted 3x quick reference sheet acrf cancer biology imaging facility institute for molecular bioscience, uq 11 april 2017 overview the leica sted 3x is an inverted compound microscope with multiple automated functions, capable of use as both a fluorescence confocal and transmitted light dic microscope 10100x magnification. Leica sp8 confocal, sted, flim system microscopy and. Leica tcs sp8 confocal microscope biological imaging facility. Webbased cloudservices portal for leica cyclone product family.
163 535 669 107 1522 719 403 1525 933 357 1548 31 1313 1362 407 719 1171 842 1570 992 600 1356 461 178 1148 704 441 362 967 937 696 1214 348 66 350 28 1434 735 368 595 1106 1217 695 135 326 297